Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Blood

Cell type

Common myeloid progenitor

NA

NA

Sample

source_name

CMP Flt3- Csf1r- Irf8-GFP- MHCII-

strain

C57BL/6

age

6-8 wks

tissue

bone marrow

cell type

common myeloid progenitor (CMP)

cell subtype

CMP Flt3- Csf1r- Irf8-GFP- MHCII-

chip antibody

K4me2 (Abcam-ab7766)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Lara-Astiaso D, Weiner A, Lorenzo-Vivas E, Zaretsky I et al. Immunogenetics. Chromatin state dynamics during blood formation. Science 2014 Aug 22;345(6199):943-9. PMID: 25103404 indexing-first chromatin immunoprecipitation

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

20988702

Reads aligned (%)

82.0

Duplicates removed (%)

6.4

Number of peaks

5131 (qval < 1E-05)

mm9

Number of total reads

20988702

Reads aligned (%)

81.8

Duplicates removed (%)

6.6

Number of peaks

5112 (qval < 1E-05)