Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Blood

Cell type

Common myeloid progenitor

NA

NA

Sample

source_name

CMP Irf8-GFP+ MHCII+

strain

C57BL/6

age

6-8 wks

tissue

bone marrow

cell type

common myeloid progenitor (CMP)

cell subtype

CMP Irf8-GFP+ MHCII+

chip antibody

K4me2 (Abcam-ab7766)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Lara-Astiaso D, Weiner A, Lorenzo-Vivas E, Zaretsky I et al. Immunogenetics. Chromatin state dynamics during blood formation. Science 2014 Aug 22;345(6199):943-9. PMID: 25103404 indexing-first chromatin immunoprecipitation

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

24091221

Reads aligned (%)

80.1

Duplicates removed (%)

12.1

Number of peaks

5375 (qval < 1E-05)

mm9

Number of total reads

24091221

Reads aligned (%)

79.8

Duplicates removed (%)

12.7

Number of peaks

5354 (qval < 1E-05)