GSM5567393: human MPCs Med1 WT – rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
MED1
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC derived pancreatic cells
NA
NA
Attributes by original data submitter
Sample
source_name
human pancreatic MPCs
cell type
HPSCs-derived MPCs (stage 4)
antibody
MED1 (Bethyl laboratories, A300-793A)
genotype
PTF1A+/+
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
MPCs were crosslinked with 1% PFA, lysed and sonicated with Covaris S220. DNA-protein complexes were isolated with antibodies, as described in Miguel-Escalada et al 2019. NEBNext Ultra II DNA library Prep Kit (New England Biolabs)