Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Embryo

Cell type

2-cell stage

NA

NA

Sample

source_name

2-cell embryos

strain

C57BL6/CBA

chip antibody

H3K4me3 (Merck Millipore, catalogue number 04-745)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was preapred from whole cell lysates of cross-linked cells. Lysates were sonicated and chromatin complexes were isolated with antibodies coupled to magnetic beads. Libraries were prepared according to Rubicon Genomics THRUPlex kit with minor modifications.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

58565093

Reads aligned (%)

72.6

Duplicates removed (%)

76.8

Number of peaks

4925 (qval < 1E-05)

mm9

Number of total reads

58565093

Reads aligned (%)

72.5

Duplicates removed (%)

76.9

Number of peaks

4902 (qval < 1E-05)