GSM5560064: EBF1 ChIP of CD19+ human primary B cells - Replicate 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
EBF1
Cell type
Cell type Class
Blood
Cell type
B-cell (CD19+)
Tissue
Blood
Lineage
primaryCells
Description
B Cell , CD19+
Attributes by original data submitter
Sample
source_name
EBF1 ChIP of CD19+ human primary B cells
cell type
CD19+ B cells
chip antibody
EBF1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The FACS sorted primary cells were crosslinked with formaldehyde, lysed and DNA was fragmented into pieces of average 300bp by sonication. Chromatin was precipitated with a specific antibody and protein A-coupled sepharose beads. After elution from the beads, chromatin was decrosslinked and purified via QIAquick PCR Purification Kit according to the manufacturer's manual. Library preparation for ChIP analysis was performed with the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (Catalog #E7370) according to the manufacturer's protocol. No size selection was used and amplification was performed using 10 PCR cycles.