GSM5550295: C33-A cells - FANCD2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
FANCD2
Cell type
Cell type Class
Uterus
Cell type
C-33 A
Primary Tissue
Cervix
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
Cervical carcinoma cells
cell type
HPV negative cervical carcinoma cells
cell line
C33-A
chip-seq antibody
FANCD2 (Bethyl, A302-174A, lot 1)
treatment
24 hour 0.2 uM aphidicolin treatment
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crossed-linked with 1% formaldehyde and chromatin was isolated and sheared to 100–500 bp DNA fragments using a Bioruptor sonicator (Diagonode) on high power settings. Chromatin samples were incubated overnight at 4°C with antibody and the resulting chromatin immunocomplexes were precipitated with blocked Dynabeads Protein G (Invitrogen), subjected to multiple wash steps and the chromatin eluted in elution buffer (50 mM Tris-HCl [pH 8.0], 10 mM EDTA [pH 8.0], 1% SDS). Chromatin was reverse cross-linked overnight at 65°C in 0.2 M NaCl, followed by RNase A and proteinase K treatment, and the DNA purified using the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries were prepared using Kapa Hyper Preps (Roche) according to the manufacturer's instructions and sequenced on the Illumina HiSeq 4000 following the manufacturer's protocol.