GSM5531324: Human colon cancer cells - WT CTCF rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
Human colon cancer cells
cell line
HCT-116
cell type
Human colon cancer cells
genotype/variation
wild type
antibody
CTCF
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% freshly prepared formaldehyde solution for 10min and then lysed with buffer containing: 50 mM Tris-HCl, pH 8; 10 mM EDTA; 1% SDS; x1 Proteinase inhibitor (Roche). Chromatin was sonicated and DNA–protein complexes were immune-purified with antibodies against CTCF (CS 2899S) DNA sample was used for library by following the manufacturer's recommendations of NEBNext® Ultra™ IIDNA Library Prep Kit (New England BioLabs, US). Index codes were added to each sample. Briefly, the genomic DNA was randomly fragmented to size of 350bp. DNA fragments were end polished, A-tailed, ligated with adapters, size selected and further PCR enriched. Then PCR products were purified (AMPure XP system), followed by size distribution by Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA), and quantification using real-time PCR. Library was then sequenced on NovaSeq 6000 S4 flow cell with PE150 strategy.