Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic Stem Cells
cell type
ES cells
cell line
v6.5
gender
male
antibody
Santa Cruz, Cat# sc-899

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were produced from soncated cell material and transcription factor associated DNA was enriched with specific antibodies. Libraries were prepared according to NEBNext kit.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
51721598
Reads aligned (%)
97.5
Duplicates removed (%)
14.0
Number of peaks
13973 (qval < 1E-05)

mm9

Number of total reads
51721598
Reads aligned (%)
97.2
Duplicates removed (%)
14.0
Number of peaks
13994 (qval < 1E-05)

Base call quality data from DBCLS SRA