Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

prostate cancer

cell line

LNCaP-abl

transfection

siMALAT1

antibody

H3K27ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Library construction was performed using the ChIP-seq DNA sample Prep Kit (Illumina) according to the manufacture’s instruction.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

22396777

Reads aligned (%)

99.7

Duplicates removed (%)

27.7

Number of peaks

41024 (qval < 1E-05)

hg19

Number of total reads

22396777

Reads aligned (%)

99.5

Duplicates removed (%)

27.9

Number of peaks

40784 (qval < 1E-05)