Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

prostate cancer

cell line

LNCaP-abl

transfection

siE2F1

antibody

H3K27ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Library construction was performed using the ChIP-seq DNA sample Prep Kit (Illumina) according to the manufacture’s instruction.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

37580933

Reads aligned (%)

99.3

Duplicates removed (%)

12.0

Number of peaks

74652 (qval < 1E-05)

hg19

Number of total reads

37580933

Reads aligned (%)

99.1

Duplicates removed (%)

12.1

Number of peaks

74253 (qval < 1E-05)