Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T-47D breast cancer cell line
cell line
T-47D
cell type
Pleural effusion from a ductal carcinoma of the breast
treatment
17β-estradiol
concentration
100nM
time
30 minutes
replicate
replicate 2
antibody
ER cocktail: Ab-10 Thermo Scientific Lab Vision, HC-20 sc-543 Santa Cruz
growth proptocol
For maintenance cells were cultured in RPMI containing 2mM L-glutamine supplemented 10% calf serum, 1mM sodium pyruvate, 1X non-essential amino acids, and 1% penicillin-streptomycin

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR/DNA, ER/DNA, of FoxA1/DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
40925137
Reads aligned (%)
84.7
Duplicates removed (%)
12.5
Number of peaks
11714 (qval < 1E-05)

hg38

Number of total reads
40925137
Reads aligned (%)
86.8
Duplicates removed (%)
11.0
Number of peaks
11751 (qval < 1E-05)

Base call quality data from DBCLS SRA