Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

ZR-75-1

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

ZR-75-1 breast cancer cell line

cell line

ZR-75-1

cell type

Ascites from a ductal carcinoma of the breast

treatment

17β-estradiol

concentration

100nM

time

30 minutes

replicate

replicate 1

antibody

ER cocktail: Ab-10 Thermo Scientific Lab Vision, HC-20 sc-543 Santa Cruz

growth proptocol

For maintenance cells were cultured in RPMI containing 2mM L-glutamine supplemented 10% calf serum, 1mM sodium pyruvate, 1X non-essential amino acids, and 1% penicillin-streptomycin

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and GR/DNA, ER/DNA, of FoxA1/DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

31438631

Reads aligned (%)

96.2

Duplicates removed (%)

9.7

Number of peaks

31707 (qval < 1E-05)

hg38

Number of total reads

31438631

Reads aligned (%)

96.8

Duplicates removed (%)

8.6

Number of peaks

31809 (qval < 1E-05)