Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7 breast cancer cell line
cell line
MCF-7
cell type
Pleural effusion from an adenocarcinoma of the breast
treatment
17β-estradiol
concentration
100nM
time
30 minutes
replicate
replicate 2
antibody
ER cocktail: Ab-10 Thermo Scientific Lab Vision, HC-20 sc-543 Santa Cruz
growth proptocol
For maintenance cells were cultured in DMEM supplemented 10% calf serum, 2mM L-glutamine, 1mM sodium pyruvate, 1X non-essential amino acids, and 1% penicillin-streptomycin

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR/DNA, ER/DNA, of FoxA1/DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
37621131
Reads aligned (%)
61.9
Duplicates removed (%)
32.3
Number of peaks
14461 (qval < 1E-05)

hg38

Number of total reads
37621131
Reads aligned (%)
63.7
Duplicates removed (%)
31.0
Number of peaks
14470 (qval < 1E-05)

Base call quality data from DBCLS SRA