GSM5482048: RING1B DAY 0 IP ChIP-seq biological replicate 2 [RING1B IP ESC D0 WT R2]; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
RNF2
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
RING1B DAY 0 IP ChIP-seq
cell type
embryonic stem cells
chip antibody
Ring1b (MBL D139-3 mouse)
timepoint
day0
treatment
untreated
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For each sample approximately 5*10^6 cells were resuspended in ice cold shearing buffer (1% Triton-X 100, 0.15 M NaCl, 1 mM EDTA, 0.5 mM EGTA, 20 mM HEPES (pH 7.6), 0.3% SDS + protease inhibitors) and transferred to three Diagenode TPX tubes. Chromatin was sheared using a Bioruptor plus: 25 cycles of 30s On/30s Off on high setting. Sheared lysates were diluted to 0.15% SDS prior to immunoprecipitation. The DNA library for sequencing was prepared from Qiagen column purified DNA. DNA fragmentation of IP and input samples was confirmed by Agilent TapeStation prior to library preparation using NEB Ultra II DNA. Libraries were sequenced as single-end, 76bp reads on the Illumina High-Seq 4000 platform (Francis Crick Institute).