Antigen

Antigen Class

TFs and others

Antigen

Hsf1

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver

strain

C57/BL6 WT

feeding

ad libitum

technique

ChIP-seq

tissue

liver

chip antibody

HSF1

time point

ZT 14

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For the HSF1 ChIP-Seq, chromatin preparation and chromatin immunoprecipitation were performed as in Rey et al., PLoS Biol 2011. The HSF1 polyclonal antibody is from Stressgen (Enzo Life Sciences). For each IP, 5ul of HSF1 antibody was used with 250ul of pre-cleared chromatin. Libraries for ultra-high throughput sequencing were prepared with the ChIP-Seq DNA sample kit (Illumina) as recommended by the manufacturer.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

31237730

Reads aligned (%)

97.2

Duplicates removed (%)

14.2

Number of peaks

917 (qval < 1E-05)

mm9

Number of total reads

31237730

Reads aligned (%)

96.9

Duplicates removed (%)

14.3

Number of peaks

1008 (qval < 1E-05)