Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Myt1l

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
TauEGFP MEFs
strain background
C57BL/6
tissue
embryonic fibroblasts
overexpressed proteins
Brn2, Ascl1, Myt1l, rtTA
chip antibody
Myt1lb (rabbit)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq were carried out following the Farnham protocol (O'Geen et al., 2011) Libraries were generated using the NEBNext ChIP-seq Library Prep Master Mix Set for Illumina

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
21614344
Reads aligned (%)
90.8
Duplicates removed (%)
16.5
Number of peaks
4230 (qval < 1E-05)

mm9

Number of total reads
21614344
Reads aligned (%)
90.5
Duplicates removed (%)
16.6
Number of peaks
4264 (qval < 1E-05)

Base call quality data from DBCLS SRA