Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
shep

Cell type

Cell type Class
Larvae
Cell type
Brain
NA
NA

Attributes by original data submitter

Sample

source_name
larval brain
cell type
larval brain
genotype
y2wct6
treatment
none
chip antibody
anti-Shep (in-house as used in Matzat et al., PLOS Gen, 2012)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq and CUT&Tag libraries were extracted by phenol-chloroform and Ampure beads after PCR amplification, respectively. ChIP-seq and CUT&Tag libraries were generated according to published protocols Matzat et al. PLOS Gen, 2012 and Hatice S Kaya-Okur, Steven Henikoff 2020. Bench top CUT&Tag. protocols.io, respectively

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm6

Number of total reads
15601452
Reads aligned (%)
44.6
Duplicates removed (%)
76.2
Number of peaks
2948 (qval < 1E-05)

dm3

Number of total reads
15601452
Reads aligned (%)
44.8
Duplicates removed (%)
74.7
Number of peaks
2549 (qval < 1E-05)

Base call quality data from DBCLS SRA