GSM5466912: LEF1 ChIP-seq from RPMI8402 cell line in basal conditions DL2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
LEF1
Cell type
Cell type Class
Blood
Cell type
RPMI 8402
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic
Attributes by original data submitter
Sample
source_name
RPMI8402 T-ALL cell line
cell type
RPMI8402 T-ALL cell line
strain
Cell line
chip antibody
LEF1, Abcam ab124271
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 0.5% formaldehyde and lysated. Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. DNA purification with GFX Kit [GE Healthcare Life Sciences Ref.28-9034-71] Samples were digested with 0.1% collagenase and single-cell suspension was stained as described. Different subpopulations were directly sorted in a total volume of 10 ml of reaction buffer and processed for obtaining DNA following manufacture's protocol. DNA amplification was performed by Long Distance PCR (LD-PCR) and the PCR-amplified DNA purified by immobilization on AMPure XP beads (Agencourt AMPure XP kit). Samples were analyzed with Agilent High Sensitivity DNA Kit (Agilent, Cat. No. 5067-4626). Covaris shearing was used for Illumina Low input sample preparation and Double last bead purification was performed to remove fragments below 200 bp. Next, samples were used to generate an Illumina sequencing library by NEBNext Ultra protocol following kit instructions. After PCR amplification of the library, the quality was checked on a Bioanalyzer and total DNA was sequenced using an Illumina HiSeq 2000 sequencer.