GSM5454401: ChIP-seq HepG2-SS IRF3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
IRF3
Cell type
Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular
Attributes by original data submitter
Sample
source_name
HepG2 cell line
cell line
liver cancer cell line
treatment
STARR-seq (SS) library transfection for 24 hours
antibody
Santa-Cruz sc-33641x
cell line
HepG2 cell line
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was performed as previously described (Sahu et al. EMBO J 2011). For analyzing genomic occupancy of TP53, cells were treated with 350 uM 5-fluorouracil (Sigma) 24 h before harvesting the cells. Briefly, fresh formaldehyde-crosslinked chromatin from GP5d cells was used to immunoprecipitate DNA using Dynal-bead coupled antibodies for TFs or histone modifications and for normal rabbit, mouse and goat IgG, followed by standard ChIP-seq library preparation for Illumina sequencing. The libraries were single-read sequenced on HiSeq4000. The immunoprecipiated DNA was end-repaired, dA tailed and adapter ligated followed by PCR enrichment of adapter-ligated ChIP DNA as described in Sahu et al. EMBO J. 2011 Illumina TruSeq library strategy