Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
BT-549
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
BT549_TNFalpha_non-specific IgG antibody
cell line
BT549
cell type
Triple negative breast cancer cells
treated with
TNFα for 6 h
chip antibody
normal rabbit IgG (sc-2027, Santa Cruz)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Standard Illumina DNA library construction ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
22270254
Reads aligned (%)
98.5
Duplicates removed (%)
65.4
Number of peaks
460 (qval < 1E-05)

hg19

Number of total reads
22270254
Reads aligned (%)
97.7
Duplicates removed (%)
67.3
Number of peaks
480 (qval < 1E-05)

Base call quality data from DBCLS SRA