Antigen

Antigen Class

TFs and others

Antigen

LDB1

Cell type

Cell type Class

Liver

Cell type

Hep G2

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

HepG2

cell type

HepG2

genotype

control cell

chip antibody

LDB1 (Abcam 96799)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA was extracted by PureLink RNA mini kit (Invitrogen, 12183020), and DNA was removed by DNase digestion (Invitrogen, 12185-010). For RNA sequencing, libraries were constructed from 1 mg total RNA by using the Truseq Stranded mRNA sample preparation kit (Illumina 20020493). ChIP-qPCR and ChIPmentatation were performed as described previously (Krivega and Dean, 2018a; Schmidl et al., 2015).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

59559237

Reads aligned (%)

46.8

Duplicates removed (%)

32.9

Number of peaks

1677 (qval < 1E-05)

hg19

Number of total reads

59559237

Reads aligned (%)

46.3

Duplicates removed (%)

34.0

Number of peaks

1610 (qval < 1E-05)