Antigen

Antigen Class

Histone

Antigen

H3

Cell type

Cell type Class

Gonad

Cell type

Oocytes

MeSH Description

Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).

Sample

source_name

MII oocyte

strain

C57BL/6N

developmental stage

MII oocyte

chip antibody

H3 antibody (Abcam, Ab1791, GR20203823-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After removing the zona pellucida, embryonic cells were washed with PBS and incubated in lysis buffer. ChIP-Seq libraries were prepared using TELP developed by PengXu et al. with slightly modification

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

19472056

Reads aligned (%)

74.3

Duplicates removed (%)

4.4

Number of peaks

64 (qval < 1E-05)

mm9

Number of total reads

19472056

Reads aligned (%)

74.2

Duplicates removed (%)

4.4

Number of peaks

57 (qval < 1E-05)