Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Neural

Cell type

Cerebellar Cortex

MeSH Description

The superficial GRAY MATTER of the CEREBELLUM. It consists of two main layers, the stratum moleculare and the stratum granulosum.

Sample

source_name

Cortex

strain

C57BL/6N x PWK

developmental stage

Cortex

chip antibody

custom-made H3K4Me3 antibody (Abmart)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After removing the zona pellucida, embryonic cells were washed with PBS and incubated in lysis buffer. ChIP-Seq libraries were prepared using TELP developed by PengXu et al. with slightly modification

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

12580049

Reads aligned (%)

78.6

Duplicates removed (%)

14.4

Number of peaks

5387 (qval < 1E-05)

mm9

Number of total reads

12580049

Reads aligned (%)

78.3

Duplicates removed (%)

14.4

Number of peaks

5379 (qval < 1E-05)