Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Embryo

Cell type

Epiblast

NA

NA

Sample

source_name

E6.5 epiblast

strain

C57BL/6N x PWK

developmental stage

E6.5 epiblast

chip antibody

custom-made H3K4Me3 antibody (Abmart)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After removing the zona pellucida, embryonic cells were washed with PBS and incubated in lysis buffer. ChIP-Seq libraries were prepared using TELP developed by PengXu et al. with slightly modification

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

9989420

Reads aligned (%)

82.1

Duplicates removed (%)

8.5

Number of peaks

25424 (qval < 1E-05)

mm9

Number of total reads

9989420

Reads aligned (%)

81.9

Duplicates removed (%)

8.5

Number of peaks

25454 (qval < 1E-05)