Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Embryo

Cell type

Blastocyst Inner Cell Mass

MeSH Description

The cluster of cells inside a blastocyst. These cells give rise to the embryonic disc and eventual embryo proper. They are pluripotent EMBRYONIC STEM CELLS capable of yielding many but not all cell types in a developing organism.

Sample

source_name

Inner cell mass

strain

C57BL/6N x PWK

developmental stage

Inner cell mass

chip antibody

custom-made H3K4Me3 antibody (Abmart)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After removing the zona pellucida, embryonic cells were washed with PBS and incubated in lysis buffer. ChIP-Seq libraries were prepared using TELP developed by PengXu et al. with slightly modification

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

35596916

Reads aligned (%)

66.5

Duplicates removed (%)

18.5

Number of peaks

17179 (qval < 1E-05)

mm9

Number of total reads

35596916

Reads aligned (%)

66.3

Duplicates removed (%)

18.5

Number of peaks

17185 (qval < 1E-05)