Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MV4;11_DMSO_ChIP
tissue source
peripheral blood
cell line
MV4;11
cell type
Mixed lineage leukemia (MLL) fusion protein (FP) leukemia cell line
treatment
0.1% DMSO
chip antibody
anti-BRD4 (E2A7X, cell signalling, lot 1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
21980330
Reads aligned (%)
72.8
Duplicates removed (%)
24.2
Number of peaks
29230 (qval < 1E-05)

hg38

Number of total reads
21980330
Reads aligned (%)
74.7
Duplicates removed (%)
22.9
Number of peaks
29366 (qval < 1E-05)

Base call quality data from DBCLS SRA