Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD21

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549-hACE2
cell line
A549-hACE2
cell type
epithelial cell
treatment
mock
chip antibody
RAD21 (Abcam, Ab992, Lot: GR214359-10)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and biotin-labelled DNA were isolated with C1 beads DNA libraries were prepared using NEB Next Ultra II DNA library prep kit for illumina according to manufactorer's instructions

Sequencing Platform

instrument_model
NextSeq 550

hg38

Number of total reads
21652424
Reads aligned (%)
81.2
Duplicates removed (%)
36.2
Number of peaks
40882 (qval < 1E-05)

hg19

Number of total reads
21652424
Reads aligned (%)
80.7
Duplicates removed (%)
36.6
Number of peaks
40656 (qval < 1E-05)

Base call quality data from DBCLS SRA