Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Others

Cell type

Lenses, Intraocular

MeSH Description

An intraocular lens permanently implanted behind the IRIS and in front of the natural EYE LENS.

Sample

source_name

newborn lens

strain background

CD-1

developmental stage

newborn (P1)

tissue

lenses

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

FAIRE-seq: The newborn (P1) lenses of CD-1 mice (Charles River Laboratories) were dissected, tissue was freshly crosslinked and homogenized. Sonicated lysates were subjected to the pheno/chloroform extraction to purify the nucleosome free regions of chromatin Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

94908999

Reads aligned (%)

87.3

Duplicates removed (%)

40.4

Number of peaks

7382 (qval < 1E-05)

mm9

Number of total reads

94908999

Reads aligned (%)

87.2

Duplicates removed (%)

40.4

Number of peaks

7150 (qval < 1E-05)