Antigen

Antigen Class

Histone

Antigen

H3K9ac

Cell type

Cell type Class

Blood

Cell type

Blood Cells

MeSH Description

The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM.

Sample

source_name

Lin-Sca1-cKit+ cells

genotype

Gfi1 36S/wt

mouse strain

C57BL/6

antibodies

H3K9Ac/H3K19Ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde. Cell lysis was performed using the following buffers: total cell lysis - 5mM PIPES pH 8, 85mM KCl, 0.5% NP-40, 1X protease inhibitor cocktail, 1mM PMSF, and nuclear lysis – 50mM Tris, 10mM EDTA, 1% SDS, 1X protease inhibitor cocktail, 1mM PMSF. After sonication (Covaris E220 sonicator), immunoprecipitation was performed using Protein A/G Dynabeads (Life Technologies) Sequencing libraries were prepared from immunoprecipitated chromatin using the TruSeq DNA kit from Illumina according to the manufacturer’s instructions and sequenced using the TruSeq PE Clusterkit v3-cBot-HS

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

17708331

Reads aligned (%)

99.5

Duplicates removed (%)

7.9

Number of peaks

389 (qval < 1E-05)

mm9

Number of total reads

17708331

Reads aligned (%)

99.5

Duplicates removed (%)

7.9

Number of peaks

396 (qval < 1E-05)