Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

MEF_WT

strain background

B6/129

genotype/variation

WT

cell type

mouse embryonic fibroblast

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Sample Prep Kit (Cat.# IP-202-1012)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

28323129

Reads aligned (%)

97.2

Duplicates removed (%)

17.5

Number of peaks

414 (qval < 1E-05)

mm9

Number of total reads

28323129

Reads aligned (%)

96.9

Duplicates removed (%)

17.5

Number of peaks

464 (qval < 1E-05)