Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

MEF_WT

strain background

B6/129

genotype/variation

WT

cell type

mouse embryonic fibroblast

chip antibody

H3K27ac (Cell Signaling Technology, D5E4, 8173)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Sample Prep Kit (Cat.# IP-202-1012)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

39205900

Reads aligned (%)

99.2

Duplicates removed (%)

5.1

Number of peaks

30917 (qval < 1E-05)

mm9

Number of total reads

39205900

Reads aligned (%)

99.1

Duplicates removed (%)

5.1

Number of peaks

31008 (qval < 1E-05)