GSM5399596: 22Rv1 48hr FOXA1 R3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
FOXA1
Cell type
Cell type Class
Prostate
Cell type
22Rv1
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
22Rv1
cell type
cancer cell line
antibody
FOXA1 (Bethyl Laboratories, A305-249A)
treatment
48hr MYCi - 10 µM
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, the cell nuclear fraction was clarified from sonicated and histone-DNA complexes were isolated with antibodies. For RNA-seq, total RNA extracts were isolated from MYCi and vehicle control treated cells. For ATAC-seq, nuclei were isolated and inspected before Tn5 tagmentation. As input for library preparation, < 1 ng – 5 ng of immunoprecipitated DNA was used. ChIPseq library preparation was carried out with KAPA Hyper Prep Kit (Cat#: KK8502). For RNAseq, 500 ng of RNA was used as input with a ribosome depletion library kit (KAPA Biosystems, Cat#: KK8560). multiplex