Antigen

Antigen Class

Histone

Antigen

H3K79me3

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

MEFs cells wildtype

cell type

mouse embryonic fibroblast

genotype/variation

wildtype

chip antibody

H3K79me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell lysates were sonicated and immunoprecipitated DNA-protein complex using antibody. ChIP DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

33863889

Reads aligned (%)

98.4

Duplicates removed (%)

6.3

Number of peaks

460 (qval < 1E-05)

mm9

Number of total reads

33863889

Reads aligned (%)

98.3

Duplicates removed (%)

6.3

Number of peaks

430 (qval < 1E-05)