GSM1833608: Input DNA GHMTd5; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast
Attributes by original data submitter
Sample
source_name
fibroblasts
strain
C57BL/6
tissue
embryo
developmental stage
E14.5
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody anti-H3K4me2. Libraries were prepared using Nugen Ovation Ultralow System Cat No 0344. Briefly, DNA was end-repaired, A-tailed, adapter ligated and amplified with 10 cycles of PCR. DNA libraries were purified using Agencourt XP Beads, quantified with the Qubit, run on a bioanalzyer tape station and clustered to an Illumina HiSEQ 2500 High Throughput Flow Cell.