Whole cortex dissected from embryonic day 15.5 (E15.5) embryos
chip antibody
none (input)
strain
CD-1
tissue
brain cortex
developmental stage
embryonic day 15.5
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cortices dissected from E15.5 embryos were fixed for 10 min with 1% formaldehyde and neutralized with glycine. The cells were lysed and the chromatin was sheared into ~300 base pair (bp) fragments. Libraries were generated from the ChIP-ed DNA and input DNA using the Illumina TruSeq kit according to the manufacturer's protocol.