GSM1829646: Head Gli2 ChIP-seq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Gli2
Cell type
Cell type Class
Embryo
Cell type
Embryonic head
NA
NA
Attributes by original data submitter
Sample
source_name
Head
developmental stage
E10.5
antibody
Gli2 (R&D Systems, AF3635)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Samples were crosslinked for 30' in 1% formaldehyde, lysed in (140mM NaCl, 0.5% NP40, 0.25% Triton-X 100, 10% glycerol, 1mM EDTA, 50mM HEPES-KOH, pH7.5) and cleared by sonication Libraries were prepared according to Illumina protocols