Antigen

Antigen Class

TFs and others

Antigen

KMT2D

Cell type

Cell type Class

Blood

Cell type

THP-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

THP-1 cells

cell type

Cultured THP-1 cells

antibody

anti-MLL4 (Santa Cruz, SC293217)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For Chip-seq, genomic DNA were pulled down by antibodies. For RNA-seq, RNA was extracted with Qiagen miRNA-ease RNA extraction kit. HICHIP DNA was collected with Arima HICHIP kit. For ChIP-seq and HICHIP, library preparation using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina. For RNA-seq, NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina was used for library prepapration. Library quality was assessed by Tapstation and Qubit concentration measurement.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

18898715

Reads aligned (%)

83.5

Duplicates removed (%)

14.8

Number of peaks

1451 (qval < 1E-05)

hg19

Number of total reads

18898715

Reads aligned (%)

81.5

Duplicates removed (%)

20.1

Number of peaks

764 (qval < 1E-05)