Antigen

Antigen Class

TFs and others

Antigen

EP300

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H9

NA

NA

Sample

source_name

H9 cell line

cell line

human embroynic stem cell line H9

chip antibody

Bethyl, A301-358A

genotype

CFIm25 KO

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked DNA-proteins were fragmented with ChIP-IT® Express Enzymatic Shearing Kit (Active Motif),cell lysates were further subject to IP with antibodies targeting specific protein. Iped DNA-protein were de-crosslinked, DNA was purified and subjected to library preparation. ChIP-seq library preparation kit (Vazyme, catlog: ND607-01) was used for preparation of ChIP-seq libraries. Libraries were sequenced with Novaseq PE150

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

51242972

Reads aligned (%)

93.3

Duplicates removed (%)

12.4

Number of peaks

599 (qval < 1E-05)

hg19

Number of total reads

51242972

Reads aligned (%)

93.0

Duplicates removed (%)

12.7

Number of peaks

289 (qval < 1E-05)