Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TBP

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA

Attributes by original data submitter

Sample

source_name
H9 cell line
cell line
human embroynic stem cell line H9
chip antibody
CST, 44059T
genotype
control

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinked DNA-proteins were fragmented with ChIP-IT® Express Enzymatic Shearing Kit (Active Motif),cell lysates were further subject to IP with antibodies targeting specific protein. Iped DNA-protein were de-crosslinked, DNA was purified and subjected to library preparation. ChIP-seq library preparation kit (Vazyme, catlog: ND607-01) was used for preparation of ChIP-seq libraries. Libraries were sequenced with Novaseq PE150

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
40751740
Reads aligned (%)
92.5
Duplicates removed (%)
9.0
Number of peaks
4389 (qval < 1E-05)

hg19

Number of total reads
40751740
Reads aligned (%)
92.2
Duplicates removed (%)
9.1
Number of peaks
4143 (qval < 1E-05)

Base call quality data from DBCLS SRA