Antigen

Antigen Class

TFs and others

Antigen

RAD21

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7 cell line

cell type

MCF7 cell line

antibody

Rad21

treatment

Estrogen + 30min

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation was performed as previously described (Komata, M., et al., Chromatin immunoprecipitation protocol for mammalian cells. Methods Mol Biol, 2014. 1164: p. 33-8.). Total RNA was extracted using TRIzol (Life Technologies) and Nucleospin RNA (Macherey-Nagel) following the manufacturers' instructions. ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (New England BioLabs).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

49775366

Reads aligned (%)

99.4

Duplicates removed (%)

5.2

Number of peaks

39455 (qval < 1E-05)

hg19

Number of total reads

49775366

Reads aligned (%)

98.8

Duplicates removed (%)

6.1

Number of peaks

39271 (qval < 1E-05)