GSM5364006: SKNMC FLI1 ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
FLI1
Cell type
Cell type Class
Neural
Cell type
SK-N-MC
Primary Tissue
Brain
Site of Extraction
Brain
Tissue Diagnosis
neuroepithelioma
Attributes by original data submitter
Sample
source_name
Ewing sarcoma cell line
cell line
SK-N-MC
chip antibody
FLI1 (Abcam, ab15289, 2 µg)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formalin for 10 min, glycine at final 200 mM was added for 5 min, cell surface was washed with ice-cold PBS three thimes, cells were lysed with the lysis buffers of the Diagenode iDeal ChIP-seq kit for transcription factors, DNA was sheared with Bioruptor Plus sonication device, cell debris was separated by centrifugation, genomic sheared DNA was added to ChIP antibodies coupled to magnetic beads. The next day, beads were washed with four washing buffers of the Diagenode iDeal ChIP-seq kit, DNA was eluted with the respective buffer and cleaned up with beads. Libraries were constructed with the Illumina TruSeq ChIP library preparation kit following the manufacturer's protocol