GSM1825764: 5dd DOX plus H3K4me2 Rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPSC derived fibroblasts
NA
NA
Attributes by original data submitter
Sample
source_name
reprogramming intermediates of hiF-T cells
treatment
DOX days 0-5
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA, including the small RNA fraction, was obtained by organic extraction followed by miRNeasy purification (Qiagen). ChIP-seq Illumina libraries were prepared and analyzed as previously described (Mikkelsen et al., 2010) starting from one million cells crosslinked in 1% PFA. ChIP-Seq libraries were sequenced with approximately 20 million 50-bp single end reads each.