Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPSC derived fibroblasts

NA

NA

Sample

source_name

immortalized human inducible fibroblasts-like cells (hiF-T)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Total RNA, including the small RNA fraction, was obtained by organic extraction followed by miRNeasy purification (Qiagen). ChIP-seq Illumina libraries were prepared and analyzed as previously described (Mikkelsen et al., 2010) starting from one million cells crosslinked in 1% PFA. ChIP-Seq libraries were sequenced with approximately 20 million 50-bp single end reads each.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

31289117

Reads aligned (%)

83.0

Duplicates removed (%)

45.7

Number of peaks

16681 (qval < 1E-05)

hg19

Number of total reads

31289117

Reads aligned (%)

82.5

Duplicates removed (%)

46.7

Number of peaks

16555 (qval < 1E-05)