Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GRHL2

Cell type

Cell type Class
Gonad
Cell type
PEO1
Primary Tissue
Ovary
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Ovarian cancer cell line PEO1
cell line
Ovarian cancer cell line PEO1
chip antibody
GRHL2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. a ChIP-seq library was constructed using NEBNext® ChIP-seq sample preparation kit (E6200, New England Biolabs) according to the manufacturer’s protocol. Size-selection was performed by gel extraction after agarose gel electrophoresis or by magnetic separation using AMPure XP beads. Multiplexing sample preparation oligonucleotide kit (#PE-400-1001, Illumina) was used for index labelling. Sample quality was analyzed using an Agilent 2100 Bioanalyzer prior to sequencing.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
25946921
Reads aligned (%)
78.0
Duplicates removed (%)
7.1
Number of peaks
13733 (qval < 1E-05)

hg38

Number of total reads
25946921
Reads aligned (%)
79.3
Duplicates removed (%)
6.2
Number of peaks
13683 (qval < 1E-05)

Base call quality data from DBCLS SRA