GSM1825677: OVCA429-GRHL2 ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
GRHL2
Cell type
Cell type Class
Gonad
Cell type
OvCA 429
Primary Tissue
Ovary
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Ovarian cancer cell line OVCA429
cell line
Ovarian cancer cell line OVCA429
chip antibody
GRHL2
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. a ChIP-seq library was constructed using NEBNext® ChIP-seq sample preparation kit (E6200, New England Biolabs) according to the manufacturer’s protocol. Size-selection was performed by gel extraction after agarose gel electrophoresis or by magnetic separation using AMPure XP beads. Multiplexing sample preparation oligonucleotide kit (#PE-400-1001, Illumina) was used for index labelling. Sample quality was analyzed using an Agilent 2100 Bioanalyzer prior to sequencing.