Antigen

Antigen Class

TFs and others

Antigen

GFP

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Daxx fl/fl Embryonic stem cell_DMSO-treated

strain

B6/129

chip antibody

GFP (A-6455, life technologies)

genotype

Daxx fl/fl; RosaCreERT (control for KO2)

treatment

DMS0

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from Micrococcal Nuclease (MNase) treated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the TruSeq ChIP Sample Prep Kit (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

32471628

Reads aligned (%)

95.9

Duplicates removed (%)

7.7

Number of peaks

422 (qval < 1E-05)

mm9

Number of total reads

32471628

Reads aligned (%)

0.0

Duplicates removed (%)

7.7

Number of peaks

430 (qval < 1E-05)