Antigen

Antigen Class

TFs and others

Antigen

NR3C1

Cell type

Cell type Class

Blood

Cell type

697

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Lymphocytic

Sample

source_name

697 (ALL cell line)

cell line

697

cell type

ALL

antibody

GR Mouse mAb (BD #611227)

treatment

PRED (10uM, 2hrs)

replicate

3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were cross-linked, lysed, and sonicated according to the ENCODE project ChIPseq guidelines (https://www.encodeproject.org/about/experiment-guidelines/). 5ug of CTCF, GR, or H3K27ac antibody was used for IP Libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

58433350

Reads aligned (%)

98.8

Duplicates removed (%)

17.7

Number of peaks

32418 (qval < 1E-05)

hg19

Number of total reads

58433350

Reads aligned (%)

98.2

Duplicates removed (%)

18.5

Number of peaks

32542 (qval < 1E-05)