Antigen

Antigen Class

TFs and others

Antigen

NR3C1

Cell type

Cell type Class

Blood

Cell type

NALM-6

Primary Tissue

Blood

Tissue Diagnosis

Leukemia

Sample

source_name

Nalm6 (ALL cell line)

cell line

Nalm6

cell type

ALL

antibody

GR Mouse mAb (BD #611227)

treatment

PRED (5uM, 6hrs)

replicate

3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were cross-linked, lysed, and sonicated according to the ENCODE project ChIPseq guidelines (https://www.encodeproject.org/about/experiment-guidelines/). 5ug of CTCF, GR, or H3K27ac antibody was used for IP Libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

37173768

Reads aligned (%)

97.9

Duplicates removed (%)

27.7

Number of peaks

26834 (qval < 1E-05)

hg19

Number of total reads

37173768

Reads aligned (%)

97.4

Duplicates removed (%)

28.3

Number of peaks

26759 (qval < 1E-05)