Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

WT DIS3 cells Mock control replicate 1

cell type

HEK293

genotype

WT DIS3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP experiments for RNA polymerase II were performed with MNase treatment and anti-RNA Polymerase II antibody (clone 8WG16, BioLegend). KAPA Hyper Prep Kit (Kapa Biosystems)

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

101105696

Reads aligned (%)

84.0

Duplicates removed (%)

26.8

Number of peaks

1066 (qval < 1E-05)

hg19

Number of total reads

101105696

Reads aligned (%)

83.5

Duplicates removed (%)

28.1

Number of peaks

898 (qval < 1E-05)