Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
WT DIS3 cells RNA Pol II CHIP replicate 1
cell type
HEK293
genotype
WT DIS3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments for RNA polymerase II were performed with MNase treatment and anti-RNA Polymerase II antibody (clone 8WG16, BioLegend). KAPA Hyper Prep Kit (Kapa Biosystems)

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
81380383
Reads aligned (%)
91.0
Duplicates removed (%)
22.2
Number of peaks
18881 (qval < 1E-05)

hg19

Number of total reads
81380383
Reads aligned (%)
90.7
Duplicates removed (%)
23.0
Number of peaks
18901 (qval < 1E-05)

Base call quality data from DBCLS SRA